(A) Electromobility supershift analysis using nuclear extracts from liver biopsies of died pregnant FHF patient, with 32P-labeled oligonucleotides harboring an NF-kB consensus sequence showing differential binding activity of NF-kB components. Liver biopsy extracts were incubated with specific antibodies (Abs) recognizing different members of the NF-kB family. Lane a, without Ab; lane b, addition of p50 Ab; lane c, addition of p65 Ab. The position of the NF-kB specific complex is indicated. The arrowheads mark the positions of additionally retarded ‘supershift’ complexes. The squares mark the unspecific complexes. The extent of supershifting as revealed by densitometry analysis are indicated below each lane and was derived in comparison to the non-shifted NF-kB complex in lane a. (B) Differential expression of p50 and p65 in liver biopsy from pregnant FHF patients who died after being admitted in the hospital; 30 µg protein from each case was separated in a 10% SDS-PAGE mini gel. After electrotransfer, the filters were consecutively incubated with p50 and p65 antibodies of NF-kB family. To confirm, equal protein loading, the filters were reincubated with a polyclonal Actin antibody. Lanes a, normal healthy pregnant women as disease controls (N); lane b and c, pregnant FHF patients who died. The increases in expression in comparison to the normal control as revealed by densitometric analysis are indicated below each lane.