Comparison of differential expression pattern of p50 and p65 in PBMC proteins from a non-pregnant FHF (lane b) and a pregnant (lane c) FHF patient who recovered back to normalcy following treatment and 2 pregnant acute viral hepatitis (AVH) patient (disease controls) (lane d and e); 30µg proteins from each case was separated in a 10% SDS-PAGE mini gel. After electrotransfer, the filters were consecutively incubated with p50 and p65 antibodies of NF-kB family. To confirm, equal protein loading, the filters were reincubated with a polyclonal Actin antibody. Lanes a, normal healthy pregnant women as disease controls (N); lane b, non-pregnant FHF patients; lane c, recovered pregnant FHF patients. The increase in expression, in comparison to the normal control, as revealed by densitometry analysis is indicated below each lane.