Figure 7From: Insulin Decreases Inflammatory Signal Transcription Factor Expression in Primary Human Liver Cells after LPS ChallengeLactate dehydrogenase (LDH) release in isolated hepatocytes. LDH release indicates that membrane integrity of hepatocytes is partly damaged during the process of isolation (day 0 = fresh isolated hepatocytes). After seeding onto collagen coated dishes, hepatocytes recover and some necrotic cells are constantly removed by a daily medium change, which is demonstrated by dropping of LDH enzyme activity to low levels in the medium after 2 days. Six different preparations of PHHs are shown.Back to article page