Table 1 Oligonucleotide sequence for real-time PCR
Sequence, 5′ to 3′ | Sequence length, bp | Concentration, mM | Annealing temperature, °C | ||
|---|---|---|---|---|---|
β-Actin | Forward Reverse | AGA GGG AAA TCG TGC GTG AC CAA TAG TGA TGA CCT GGC CGT | 138 | 3 | 62 |
GAPDH | Forward Reverse | GCG GGG CTC TCC AGA ACA TCA T CCA GCC CCA GCG TCA AAG GTG | 301 | 3 | 64 |
IL-1β | Forward Reverse | CAG GCC GCG TCA GTT GTT GT CCG GAG CGT GCA GTT CAG TG | 195 | 3 | 60 |
TNF-α | Forward Reverse | CGC CAC CAC GCT CTT CTG C ACG GCG ATG CGG CTG ATG | 354 | 2 | 64 |
IL-6 | Forward Reverse | CCC AGT ACC CCC AGG AGA AGA GTT GGG TCA GGG GTG GTT ATT G | 426 | 3 | 60 |
IL-10 | Forward Reverse | GAC CCA GCC CCT TGA GAA ACC T GCC CCA AGC CCA GAG ACA AGA T | 359 | 3 | 64 |
IFN-γ | Forward Reverse | GGG TTC TCT TGG CTG TTA CT TTG GCT CTG CAT TAT TTT TC | 166 | 3 | 58 |
STAT-5 | Forward Reverse | TCA TCA TCG AGA AGC AGC C TTC CGT CAC AGA CTC TGC AC | 317 | 3 | 60 |
α-SMA | Forward Reverse | CGT GGC TAT TCC TTC GTT AC TGC CAG CAG ACT CCA TCC | 174 | 3 | 57 |
CD68 | Forward Reverse | AGC CCA GGA TTC ACC AGT T GGT TTT GTT GGG GTT CAG TAC | 144 | 3 | 57 |
Albumin | Forward Reverse | GCTGCCATGGAGATCTGCTTGA GCAAGTCAGCAGGCATCTCATC | 174 | 3 | 53 |
CK18 | Forward Reverse | CCCGCTACGCCCTACAGA GCGGGTGGTGGTCTTTTG | 248 | 3 | 53 |