Figure 5

Immune responses of mice treated by weekly injection of hASA. (A) Lethality during treatment for up to ten weeks using 20 mg/kg (conventional ASA−/− mice (closed triangles, n = 20] and wildtype controls [open squares, n = 5)) or 15 mg/kg hASA (transgenic ASA−/− mice [closed circles, n = 20)). As a control, some conventional ASA−/− mice were treated with buffer (open triangles, n = 10). Asterisk-due to severe side effects treatment was terminated after four injections. (B) α-hASA antibody titers of mice treated for up to 17 weeks with 2.5 mg/kg hASA. Same legend as in (A). hASA-C69S serum concentrations of transgenic ASA−/− mice: < 0.01–1.70 µg/mL. Bars indicate means ± SDs, n = 4–5.