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Figure 2 | Molecular Medicine

Figure 2

From: Clinical Significance of Telomerase Activity in Peritoneal Disseminated Cells: Gastrointestinal Cancers

Figure 2

Relative levels of telomerase activity (RTQ-TRAP assay) in disseminated cells isolated and immunomagnetically sorted from the peritoneal washing fluid of gallbladder cancer patient. Thin lines present the standard amplification curves of the telomerase activity products from known number of cancer cells (five serial 1:10 dilutions of 104 PC-3 cells; the green curve corresponds to the highest protein concentration from 104 PC-3 cells; no amplification was seen from one PC-3 cell: brown curve below the threshold line, Ct). Thick magenta line presents TA in clinical sample that was automatically calculated by the Opticon MJ Research software and was equivalent to 3,395 PC-3 cells. Quantification of the TA levels in clinical specimens in relation to the common cultured cancer cells as a standard reference material allows an accurate comparative analysis of different clinical specimens, different experiments, and data obtained from different laboratories.

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