Figure 1

Identification of Mas protein in platelets. (A) Western blot of platelet lysates from Wistar rats were probed with a polyclonal anti-Mas mouse antibody, revealing a single band corresponding to the Mas protein (33 kDa). A positive control sample of Wistar rat testis was also applied to each lane of the the gel. Blots shown are representative of at least three independent experiments controlled for load and detectable protein. For each blot, a total of 30 µg of protein was applied to the gel. (B) Ang-(1-7) binding to platelets. Ang-(1-7) at 10⁻⁵ mol/L and A779 at 10⁻⁵ mol/L displaced FAM-Ang-(1-7) at 2 × 10⁻⁹ mol/L. Graphic shows the total fluorescence captured. (C,D,E) Confocal photomicrographs of the FAM-Ang(1-7) binding on rat platelets; (C) total binding, (D) nonspecific binding, and (E) Mas-antagonist A-779 displacement of the FAM-Ang-(1-7) binding. **P < 0.01, significantly different from the respective control group (Mann Whitney test). Each column represents the mean ± SEM of relative fluorescence in arbitrary unity (A.U.) from four experiments.