Figure 2
From: The Antithrombotic Effect of Angiotensin-(1-7) Involves Mas-Mediated NO Release from Platelets
Ang-(1-7) binding on platelets is absent in Mas−/− mice. (A) Total fluorescence captured; Ang-(1-7) at 10−5 mol/L displaced FAM-Ang-(1-7) at 2 × 10−9 mol/L in Mas+/+ mouse platelets. Ang-(1-7) binding was absent in Mas−/− mice. (B,C,D,E) Confocal photomicrographs of the FAM-Ang(1-7) binding on Mas+/+ and Mas−/− mouse platelets. Total binding (B) and nonspecific binding (C) in Mas+/+ mouse platelets. Total binding (D) and nonspecific binding (E) in Mas−/− mouse platelets. (F) In a digital magnification made from image B, focus planes show the inside layers from the middle or proximal to the surface of cells. The arrows shows FAM-Ang-(1-7) binding to the perimeter of platelets. Fluorescence was also observed inside platelets, suggesting internalization **P < 0.01, significantly different from the respective control group (Mann Whitney test). Each column represents the mean ± SEM of relative fluorescence in arbitrary unity (A.U.) from four experiments.