Table 1 Oligonucleotides used for qPCR
From: Neutrophils in Cystic Fibrosis Display a Distinct Gene Expression Pattern
Gene | Sense oligonucleotide | Antisense oligonucleotide |
|---|---|---|
GAPDH | 5′-GAGTCAACGGATTTGGTCGT-3′ | 5′-TTGATTTTGGAGGGATCTCG-3′ |
Thrombospondin-1 | 5′-AAAGGATAATTGCCCCAACC-3′ | 5′-CGGTCTCCCACATCATCTCT-3′ |
G-CSF | 5′-GCTTGAGCCAACTCCATAGC-3′ | 5′-TCCCAGTTCTTCCATCTGCT-3′ |
CXCL10 (IP-10) | 5′-AGGAACCTCCAGTCTCAGCA-3′ | 5′-CAAAATTGGCTTGCAGGAAT-3′ |
IKKε | 5′-GTGCACAAGCAGACCAGTGT-3′ | 5′-GCCCTTGGCAGTGTTGTAAT-3′ |
CCL17 (TARC) | 5′-CTGCAAAGCCTTGAGAGGTC-3′ | 5′-CATGGCTCCAGTTCAGACAA-3′ |
IL-10Rα | 5′-GGATTCACTGAGGGGAGACA-3′ | 5′-GCAGCAAAGTGAGGATGTGA-3′ |