Figure 4

Adenovirus-mediated overexpression of esRAGE restores diabetes impairment of angiogenic response. Control or streptozotocin-induced diabetic C57BL/6J mice were used to examine the effect of esRAGE overexpression in vivo. VEGF (300 ng/mL) and either Ad-esRAGE or Ad-LacZ were suspended in matrigel (5 × 10⁸ pfu/mL), and the matrigel plugs were implanted subcutaneously into the abdominal midline of 13-week-old mice. The plugs were removed two weeks later and processed for histochemical analysis. _Bars represent 50 µm. Summary of the quantitative analyses (n = 5 for each group) is shown in the right panel. Data are shown as mean ± SD. *P < 0.05 vs. Ad-Lac Z infected control group, **P < 0.05 vs. Ad-Lac Z infected group, ANOVA with multiple comparison (Scheffe type). Modified from (30).