Table 4 Factors affecting transfection efficiency with nonviral vectors.
From: Nonviral Vector Gene Modification of Stem Cells for Myocardial Repair
Nonviral vector formulation | |
| Â | Size of the lipid particle or liposome Type of the lipid (saturated or unsaturated, branched or unbranched carbon chain, etc.) Residual charge or zeta potential on the lipid/DNA complex Size of the lipid/DNA complex Ratio between lipid and DNA Protective effect of lipid formulation against nuclease degradation Stability under different storage conditions Incubation time and temperature |
Biological | |
| Â | Type of cell Degree of cell confluence at the time of transfection Presence or absence of serum Nature of target tissue Route of administration Nucleocytoplasmic trafficking of DNA |