Figure 5
From: The Ubiquitin-Proteasome Pathway Mediates Gelsolin Protein Downregulation in Pancreatic Cancer
Correlation of gelsolin protein levels with pancreatic cancer lymph node metastasis. (A) Representative Western blot analysis of pancreatic cancer tissues with and without lymph node (LN) metastasis. Arrow at 90 kDA indicates gelsolin. Arrow at 54 kDA indicates α-tubulin. (B) Band intensity was quantified using Quantity One software. α-Tubulin serves as an internal control. (C) Gelsolin protein in the pancreatic cancer tissues with and without lymph node metastasis is immunoprecipitated (IP) by anti-gelsolin antibody, and then Western blot (WB) analyzes the ubiquitin-tagged gelsolin with anti-ubiquitin antibody. Bracket indicates gelsolin-Ub conjugates.