Figure 2From: Pancreatic Expression and Mitochondrial Localization of the Progestin-AdipoQ Receptor PAQR10Developmental expression of the PAQR10 gene. Total RNA was extracted from mouse embryos at e10.5 and e12.5 or from dissected pancreas at e13.5, e15.5, e17.5, and newborn stages. RNA was (+) or was not (−) reverse transcribed and subjected to PCR with specific oligonucleotides for PAQR10, Ngn3, PAQR1, PAQR9, PAQR11, and β-actin (internal control). Amplified products were analyzed on 1.5% agarose gels.Back to article page