Figure 5

Decreased insulin-induced tyrosine phosphorylation of insulin receptor substrate-1 (IRS-1) and insulin receptor (IR) in the skeletal muscle following trauma and hemorrhage. Rats were subjected to trauma (T) or trauma and hemorrhage (TH) as described in Figure 4 and tissues lysates were subjected to Western blotting with antibodies specific for phospho-IRS-1 tyrosine 612 (P-IRS-1 Y612) and for phospho-IR tyrosine 972 (P-IR Y972). (A) Representative Western blots from TH30′, TH60′, and TH90′ are presented, and (B) autoradiographs were quantified by scanning densitometry; the data are presented as mean ± SEM fold change of P-IRS-1 (Y612) by insulin of three rats (n = 3) in each group. (C) Representative Western blots from TH30′, TH60′, and TH90′ are presented and (B) autoradiographs were quantified by scanning densitometry; the data are presented as mean ± SEM fold change of P-IR (Y972) by insulin (n = 3 in each group). T0′ with no insulin treatment was arbitrarily set to 1. #P < 0.05 compared with T0′; *P < 0.05 compared with T only group at the same time point.