Figure 6

Insulin-induced Phospho-Akt (P-Akt) in extensor diaphragm and digitorum longus muscles and cardiac muscle following trauma and hemorrhage. Rats are subjected to trauma alone (T) or trauma and hemorrhage (TH) as described in Figure 1 except (A) diaphragm muscle, (B) extensor digitorum longus muscle, and (C) cardiac (left ventricle) muscle were analyzed at the designated time points following the hemorrhage. Tissue lysates were subjected to Western blotting with antibodies specific for P-Akt serine 473 (S473) or total Akt as indicated. Representative Western blots of at least three separate experiments for each tissue are presented for TH60′ in (A) and (B) and identical data was obtained at TH90′ (TH90’ not shown). In (C) both TH60′ and TH90′ are presented.