Figure 2

Immunohistochemical localization of EPO in the spinal cord. (A) Representative picture showing the pattern of EPO staining along the spinal cord in chronic EAE, 20 d after immunization. The more caudal part of the spinal cord, representing the lumbar tract, is on the left of the picture, and the thoracic regions are on the right. (B–C) Higher magnification pictures showing EPO in the ventral horns of the lumbar spinal cord in EAE (B) and a control mouse (C). (D–F) Nissl staining (D), EPO (E), and their colocalization (F), in the anterior horns of the lumbar spinal cord in EAE. (G–I) GFAP (G), EPO (H), and the merging field obtained by the overlapping of both signals (I), in the anterior horns of the lumbar spinal cord in EAE. Scale bars: A, 500 µm; B–C, 80 um; D–F, 50 µm; G–I, 60 µm.