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Figure 5 | Molecular Medicine

Figure 5

From: Endogenous Erythropoietin as Part of the Cytokine Network in the Pathogenesis of Experimental Autoimmune Encephalomyelitis

Figure 5

EPO expression is downregulated by inflammatory cytokines in vitro. Kelly neuroblastoma cells were incubated for 24 h with 200 µM deferoxamine mesylate (DFX) in the presence or absence of IFN-γ (A), or TNF at the indicated concentrations (10 or 100 ng/mL), with or without preincubation with 10 U/mL IFN-γ (B), and EPO secretion was measured by ELISA. Rat glial cells were incubated for 24 h with 500 µM dimethyloxalylglycine (DMOG) and the indicated concentrations of IFN-γ (C) or TNF (D), and EPO expression was analyzed by real-time RT-PCR. Results are the mean ± SEM of triplicate samples tested in duplicate. *P < 0.05; **P < 0.01; ***P < 0.001 versus DFX or DMOG alone; #P < 0.01 versus DFX + IFN-γ; Student ttest.

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