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Figure 3 | Molecular Medicine

Figure 3

From: Chronic Lymphocytic Leukemia Cells Recognize Conserved Epitopes Associated with Apoptosis and Oxidation

Figure 3

Differential binding of CLL mAbs to healthy versus apoptotic cells. (A–C) Viable Jurkat T cells were fixed, permeabilized, and exposed to SYTOX Orange and mAb 114 (50 µg/mL) plus FITC-labeled (Fab′)2 goat anti-human IgG. (D–G) Reacivity of mAb 114 with surfaces of camptothecin-treated Jurkat T cells was anayzed using cells that were not permeabilized and co-stained with SYTOX Orange and Annexin V-Alexa Fluor 647; reactivity with membranes of apoptotic blebs without (H–K) and with (L–O) DNA was determined similarly. Blue: nucleic acid binding by SYTOX Orange; red: membrane Annexin V binding; green: CLL mAb binding; yellow: co-localization of Annexin V and mAb binding.

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