Western blot analysis of the hNET protein expression in PANC-1 cells infected with GLV-1h99. PANC-1 cells were either mock-infected or infected with GLV-1h68 or GLV-1h99 at an MOI of 0.5 for 6, 12, 24, and 48 h, respectively. The hNET protein was detected by Western blot analysis using monoclonal anti-hNET antibody. Only GLV-1h99-infected cells expressed the hNET protein, but cells either mock-infected or infected with GLV-1h68 did not. The arrows indicate the different forms of the hNET protein detected in GLV-1h99-infected cells. The molecular weight marker bands (in kDa) are shown on the left.