Figure 5

Analysis of IL-2 and IL-10 transcript levels after islet allograft by quantitative RT-PCR. On d 5, total RNA was purified from liver sections and analyzed by quantitative RT-PCR for rat GAPDH, IL-2, and IL-10. Target gene expression in each sample was quantified using the ΔΔCt method and normalized to the levels of the housekeeping gene, GAPDH, Normalized target gene expression in experimental groups was expressed relative to that in control rats (*P < 0.005, **P < 0.05 versus control).