Table 1 Primer sequences and PCR conditions for MSP analysis.
Primer name | 5′–3′ Primer sequence forward | 5′–3′ Primer sequence reverse | Genomic positiona | AT,b °C | Product size, bp |
|---|---|---|---|---|---|
CDH13-U | 5′-TTGTGGGGTTTGTTTTTTGT | 5′-AACATTTTCATTCATACACACA | −267 | 62 | 243 |
CDH13-M | 5′-TCGCGGGGTTCGTTTTTCGC | 5′-GACGTTTTCATTCATACACGCG | −267 | 62 | 243 |
MGMT-U | 5′-TTTGTGTTTTGATGTTTGTAGGTTTTTGT | 5′-AACTCCACACTCTTCCAAAAACAAAACA | −46 | 59 | 93 |
MGMT-M | 5′-TTTCGACGTTCGTAGGTTTTCGC | 5′-GCACTCTTCCGAAAACGAAACG | +26 | 59 | 81 |
RARβ-U | 5′-TTAGTAGTTTGGGTAGGGTTTATT | 5′-CCAAATCCTACCCCAACA | −69 | 59 | 233 |
RARβ-M | 5′-GGTTAGTAGTTCGGGTAGGGTTTATC | 5′-CCGAATCCTACCCCGACG | −71 | 59 | 235 |
RASSF1A-U | 5′-GG TTTTGTGAGAGTGTGTTTAG | 5′-CACTAACAAACACAAACCAAAC | −73 | 59 | 169 |
RASSF1A-M | 5′-GGGTTTTGCGAGAGCGCG | 5′-GCTAACAAACGCGAACCG | −73 | 64 | 169 |