Increased thrombus formation in SHR is associated with lower ACE2 activity in thrombi. (A) FeCl2-induced thrombus weight in SHR and WKY; (B) ACE2 activity in thrombi from SHR and WKY; (C) Representative Western blotting analysis of ACE2 levels. Data were normalized by using β-actin. (D) Quantification of Western blotting data. *P < 0.05 versus WKY (unpaired Student t est, n = 8 to 10). A.U., arbitrary units.