Figure 3

FVIIa signaling is mediated through Egr-1 and depends on TF and PAR-2 in keratinocytes. (A) Egr-1 mRNA levels in keratinocyte in response to addition of murine FVIIa. (B) MIP-2 mRNA levels from keratinocytes obtained from WT (filled bars) and Egr-1^−/− (unfilled bars) mice after various combinations of mFVIIa (50 nmol/L) and LPS (10 µg/mL) were added to the culture. The Student t test was performed to compare WT and Egr-1^−/− keratinocytes within each treatment group. (C–D) Egr-1 and MIP-2 are downstream targets of PARs signaling in keratinocytes. Expression levels of Egr-1 and MIP-2 are measured in WT keratinocytes before and after stimulation with either PAR-1/AP 100 µmol/L, or PAR-2/AP 100 µmol/L. (E, F) FVIIa-induced signaling in keratinocyte is mediated by TF and PAR-2. Expression levels of Egr-1 (E) and MIP-2 (F) in keratinocyte derived from WT (filled bars), PAR-1^−/− (open bars), PAR-2^−/− (horizontal striped bars) and TF^−/−hTF (tg) (vertical striped bars) mice, before (− mFVIIa) and after (+ mFVIIa) addition of 50 nmol/L mFVIIa for 2 h. *P < 0.05, N ≥ 3 for each genotype.