Figure 5

Egr-1 is a critical mediator in wound healing and cell migration. (A) Kinetics of skin wound healing in WT (solid line) and Egr-1^−/− (dashed line) mice. The values represent the mean ± SEM (N = 4 for WT and N = 6 for Egr-1^−/− mice). *P < 0.05 at d 5, 7, 9, 11, 13 and 15. (B) Primary keratinocytes (top chamber) from Egr-1^−/− mice (unfilled bars) showed significantly reduced migration toward EGF (20 ng/mL in the bottom chamber) compared with WT keratinocytes (filled bars). (C, D) Inflammatory cell recruitment in WT (filled bars) and Egr-1^−/− mice (unfilled bars) after thioglycollate challenge. (C) Total peritoneal leukocytes after thioglycollate challenge from WT and Egr-1^−/− mice. Differential counts of peritoneal neutrophils (D) and macrophages (E). *P < 0.05, N = 3−8.