Figure 2
From: Interferon γ-Induced Human Guanylate Binding Protein 1 Inhibits Mammary Tumor Growth in Mice
Effect of hGBP-1 expression on tumor growth in a syngeneic mammary carcinoma mouse model. (A) Tumor growth was monitored in Balb/c mice injected with 1 × 106 TS/A cells transfected with either pUHD10.3-GBP (▲, ◯) or the parental vector pUHD10.3-Hyg (▼, ■). At d 7 after injection, animals were divided into two groups (n = 20) given either doxycycline (+ dox) or saline (− dox). Mean tumor volumes (± SEM) of individual animals are indicated. Animals that did not develop a tumor at the end of the experiment or died due to unrelated reasons were excluded. Statistical significance (P values) was determined applying a Student t test comparing the tumor doubling times of doxycycline-treated versus nontreated animals. (B) hGBP-1-specific immunohistochemical analysis of tumors derived from pUHD-GBP-transfected TS/A cells treated with doxycycline (a, b) or saline (c). No unspecific staining was observed in tumors from doxycycline-treated animals that had been injected with TS/A cells harboring the control vector pUHD (d).