Figure 5
From: Interferon γ-Induced Human Guanylate Binding Protein 1 Inhibits Mammary Tumor Growth in Mice
Analysis of tumor angiogenesis. (A) Immunohistochemistry. Consecutive sections of hGBP-1-expressing (TS/A-GBP + dox) and nonexpressing (TS/A-Hyg + dox, TS/A-GBP − dox) tumors were stained for the presence of hGBP-1 (α-hGBP-1, brown cytoplasmic staining) and CD31 (α-mCD31), a specific marker of endothelial cells (red staining). (B) Biochemical determination of hemoglobin contents. The amount of hemoglobin, normalized to total protein amounts, was determined in frozen tissue (n = 8) of hGBP-1-expressing (TS/A-GBP+) and nonexpressing (TS/A-GBP−) tumors. Statistical significance (P = 0.0032) of the encountered differences was shown by applying an unpaired Student t test.