Figure 4
From: Immunomodulatory Drugs Regulate HMGB1 Release from Activated Human Monocytes
Methotrexate or colchicine had no effect on HMGB1 and TNF release. Effects of methotrexate on HMGB1 and TNF release and on cell viability (A–C), and effects of colchicine on HMGB1 and TNF release and on cell viability (D–F), are demonstrated. Human primary monocytes were pretreated with methotrexate or colchicine for 1 h and subsequently stimulated with LPS/IFNγ for 24 or 7 h. Secretion of HMGB1 and TNF was detected by ELISPOT. Data from at least three experiments were normalized by denoting the number of spots from LPS/IFNγ stimulated cells as 100% and subsequently calculating the effect achieved by addition of the drug; P values were calculated by Kruskal-Wallis non-parametric ANOVA test and found to be nonsignificant. Cell viability was assessed by Annexin-V staining.