Figure 2

Rapamycin treatment inhibits the phosphorylation of mTOR and mTOR target proteins. (A) Rapamycin (10 µmol/L) reduced levels of phospho-mTOR, with a phospho-mTOR/total median reduction of 47% (P = 0.026). (B) Levels of phosphorylation of mTOR targets phospho-p70S6K (phospho-p70S6K/total median reduction of 15%, P= 0.026) and (C) phospho-4EBP1 (phospho-4EBP1/total median reduction of 29%, P= 0.002) were also decreased, confirming the inhibition of the mTOR pathway (data shown are representative of experiments done with six or seven different DA FLS cell lines with 30 min of stimulation; Mann-Whitney test [see Methods]). D = DMSO; R = rapamycin.