Representative images of periodic acid Schiff (PAS) staining from rats in the normal (A), no treatment (B), Ad-GFP (C), Ad-ACE2 (D), ACEI (E) and Ad-ACE2 + ACEI (F) groups, respectively. In the no treatment and Ad-GFP groups, large focal PAS-positive deposits were evident (arrows) in the mesangium, indicating mesangial expansion. Exudative lesions with fibrin-cap formation (arrowhead) in glomeruli were also observed in the no treatment and Ad-GFP groups. (G) Quantitative analysis of glomerulosclerosis. Bar = 50 µm. *P < 0.05, #P < 0.01 versus normal group; §P< 0.01 versus no treatment group.