The effect of 24-h fasting of mice on the (A) processing of hepatic procaspase-3 (p32) to the active form (p17) by Western blot and (B) hepatic DNA laddering induced by APAP (530 mg/kg; 5 h). The effect of 24 h-fasting of mice on the serum level of (C) K18 apoptotic fragments (pmol/mL), (D) total HMGB1 content (ng/mL) and (E) ALT activity (U/L) after APAP treatment (530 mg/kg; 5 h). (F) The effect of APAP (530 mg/kg) on hepatic ATP content was determined over 0–5 h in fed (solid line) and fasted mice (dotted line). Figures are representative and data is given as mean ± SD of six mice per group carried out in three independent investigations. Statistical significance was assigned as *P < 0.05, **P< 0.01 relative to time-matched controls.