Figure 4

The effect of glucose and glycine administration (1000/100 mg/kg; 1 h pre-APAP) to fasted male CD-1 mice on APAP-induced (530 mg/kg; 5 h) apoptosis and necrosis. (A) Processing of hepatic procaspase-3, (B) hepatic DNA laddering, (C) hepatic GSH depletion (nmol/mg), (D) serum caspase-mediated K18 fragment level (pmol/mL), (E) total serum HMGB1 content (ng/mL) and (F) serum ALT activity (U/L). Figures are representative and data is given as mean ± SD of six mice per group carried out in three independent investigations. Statistical significance was assigned relative to vehicle-treated controls or between APAP alone and APAP + glucose/glycine (*P < 0.05, **P < 0.01 and ***P < 0.005).