Figure 2

Proliferation of B16-LacZ cells cocultured with mesenchymal stromal cells (MSCs). (A) Standard curve correlating LacZ expression with cell numbers. B16-LacZ cells were seeded on 96-well plates at different cell numbers. After 24 h, β-galactosidase protein levels were analyzed in each well by measuring the absorbance at 595 nm using a microplate reader, as described in Materials and Methods. (B) B16-LacZ cells were cultured with MSCs at a 1:1 ratio. β-Galactosidase protein expression was measured at the indicated time points (n = 5; **P < 0.01). (C) B16-LacZ cells were incubated with MSCs at different ratios. β-Galactosidase protein expression was measured after 48 h (n = 8; **P < 0.01). OD 595, optimal density absorbance at 595 nm.