Figure 5
From: Mesenchymal Stromal Cells Promote Tumor Growth through the Enhancement of Neovascularization
Analysis of B16-LacZ tumors in xenograft tumor models in the presence and absence of co-injected MSCs. (A) Photographs of tumors generated by injection of B16-LacZ alone or by co-injection of B16-LacZ cells with MSCs. On day 21, tumors were stained with hematoxylin and eosin, a Ki-67 antibody and a CD31 antibody. (B–D) Quantitative analyses of tumor angiogenesis. When tumors reached approximately 1 cm3, they were removed, fixed and stained with a CD31 antibody (B). Microvessel density in tumor cryosections was analyzed by visualization of CD31 staining at 200× magnification. Microvessel density was quantified using the ImageJ software (n = 10; *P < 0.05; **P < 0.01). (C) B16-LacZ. (D) LLC.