HIV-pulsed DC-primed T cells with decreased TNF-α, IFN-γ and IL-2 secretions. Day-8 supernatants were harvested and analyzed by IL-2, TNF-α and IFN-γ using a commercial Bio-Plex™ Cytokine Luminex assay, and the results were normalized. (A) A representative figure for TNF-α, IFN-γ and IL-2 secretion in MLR supernatants. (B) Normalized TNF-α (n = 7, P < 0.005), IFN-γ (n = 6, P < 0.003) and IL-2 expression values (n = 6, P < 0.05). P < 0.005 and P < 0.001, paired t test.