Figure 1

A murine model of glaucoma filtration surgery. Schematic diagram depicting the surgical process in the mouse eye. The conjunctiva was dissected so as to expose the sclera and a small filtration subconjunctival space was created by running the surgical scissors underneath the dissected conjunctiva (A). An incision was then made with a 30-gauge needle through the sclera into the anterior chamber of the eye to create a fistula (B) so as to allow aqueous humor to exit from the anterior chamber and into the subconjunctival space (C). The final surgical step involved suturing the dissected conjunctiva over the newly created fistula (D). For the MMC treatment arm, 0.4 mg/mL MMC was applied between the sclera and the dissected conjunctiva using a small section of surgical sponge for 1 min (E). After the sponge was removed, the area was irrigated with 0.9% sodium chloride before cannulation of the sclera (B) and the final suturing of the conjunctiva (D). This figure is modified from a similar figure published previously (21).