Figure 5
From: Validation of the Glaucoma Filtration Surgical Mouse Model for Antifibrotic Drug Evaluation
MMC reduced collagen deposition at the surgical site. (A,C) Hematoxylin and eosin (H&E) staining revealed a flattened conjunctiva at the day-28 control operated site (boxed area) whereas the MMC-treated operated site subconjunctival space was expanded with an almost clear matrix (boxed area); scale bar, 500 µm. (B,D) Higher magnification of insets (boxed areas in A and C respectively); scale bar, 100 µm. (E,G) Picrosirius red-stained sections of the same eyes at low magnification and visualized by brightfield microscopy; scale bar, 100 µm. (F,H) Higher magnification of insets (boxed areas in E and G respectively) and polarization microscopy revealed differences in the collagen matrices of the control versus MMC-treated eye; scale bar, 100 µm. (I,J) Immunofluorescence analysis of sections from the same eyes with a collagen I-specific antibody; scale bar, 10 µm. S, sclera.