Figure 7
From: Validation of the Glaucoma Filtration Surgical Mouse Model for Antifibrotic Drug Evaluation
Recruitment of inflammatory cells to the operated site of control versus MMC-treated eyes. (A,B) Immunostaining for CD45+ leukocytes revealed the presence of these cells in the conjunctival epithelium and episclera region of the control operated area (A, arrowheads) while in the MMC-treated operated site, they were detected mainly in the posterior scleral cell layers (B, arrowheads). (C,D) Immunostaining with the CD11b antibody showed that inflammatory cells were present in the episclera area of the control operated site (C, arrowheads) while CDIIb-positive inflammatory cells were absent in the MMC-treated operated area. (E,F) α-SMA-positive cells present in the episcleral area of the control operated site may represent pericytes surrounding vascular vessels (E, arrowheads) which in the MMC-treated eye were present only in the posterior scleral cell layers at the surgical site (F, arrowhead). CE, conjunctival epithelium; S, sclera. Scale bar, 10 µm.