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Figure 8 | Molecular Medicine

Figure 8

From: Validation of the Glaucoma Filtration Surgical Mouse Model for Antifibrotic Drug Evaluation

Figure 8

MMC effects on mouse conjunctival cell proliferation, apoptosis and collagen Iα1 expression. (A) Cell growth was monitored continuously using the RTCA SP instrument. The cell index profiles of untreated cells (dotted black line) and MMC-treated cells (red line) reflect the logarithmic growth phase and response to treatment respectively. The cell index values for MMC-treated cells did not demonstrate a logarithmic growth phase that was measured for the control cells indicating that the treated cells did not proliferate. (B) Cells were treated with MMC for 1 min and maintained in culture medium for 72 h before staining with DAPI (blue) and anti-Ki67 antibody (red). Selected cells that are positive for Ki67 are indicated by arrowheads; scale bar, 100 µm. (C) Cells were treated as in (B) and analyzed by TUNEL staining (green). Nuclei were visualized by DAPI staining (blue). Cells positive for TUNEL staining are indicated by arrowheads; scale bar, 100 µm. (D) Cells were treated the same as in (B) and assayed for collagen Iα1 mRNA abundance by real-time quantitative PCR. MMC treatment significantly reduced the amount of collagen Iα1 mRNA by 40% compared with untreated control cells (P = 1.8 × 10−10). Bars represent the SD. Data shown is representative of three independent experiments.

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