Figure 1
From: Retroviral Insertional Mutagenesis Can Contribute to Immortalization of Mature T Lymphocytes
Retroviral vector design and phenotype of transduced primary T lymphocyte cultures. (A) A gammaretroviral vector coexpressing LMO2 and EGFP (MP91-LMO2) as well as a control vector expressing EGFP only (MP91-EGFP) were used in this study. IRES, internal ribosome entry site; wPRE, Woodchuck hepatitis virus posttranscriptional regulatory element. (B) Transduction efficacies (EGFP expression) as well as CD4 and CD8 expression levels were determined by flow cytometry 2 d after transduction and are shown for the vectors MP91-EGFP and MP91-LMO2.