Figure 2
From: Rapamycin Ameliorates Dystrophic Phenotype in mdx Mouse Skeletal Muscle
RAPA treatment lowers muscle fiber necrosis in mdx muscles. Six wk after treatment of 6-wk-old mdx mice by either rapamycin (RAPA)-containing microparticles (Rapa Bead or RB) or RAPA-containing drinking water (Rapa H2O or RH), tibialis anterior (TA) muscles of all of the mice and diaphragm (Dia) muscles of Rapa H2O-treated mice and control mice were collected for analysis. Control groups included age-matched wild-type C57BL/10 (B10) mice, untreated mdx (Unt) mice and blank bead (BB)-treated mice. Muscle sections incubated with isotype control antibody (Iso) in place of the primary antibody serve as negative controls for immunohistochemistry. Graphs show data of TA (A) and Dia (B) muscle tissues. Graphs show average number of necrotic muscle fibers per TA or Dia muscle cross-sections ± SE. n = 5 for RAPA-treated and B10 groups and n = 4 for untreated group. *P < 0.05; **P < 0.01. Representative sections of TA and Dia muscle incubated with an Alexa 488-conjugated anti-mouse IgG is shown (C).