Figure 3

Validation of GSK-3β kinomics array. signals Western blots, performed for SER-9 phosphorylated GSK-3β (A and B) Phosphorylation of serine 9 results in GSK-3β deactivation To compare Western blot data with kinase activity data, time point/sham ratios for both data sets were determined Owing to the inhibitory outcome of the Western blot phosphorylation data, ratios of this set were inversed. Inversed Western blot ratios were compared with the time point/sham ratios of the kinase activity data from the kinomics array (C) These activity patterns overlap, showing consistency between Western blot and kinase activity array methods Data are mean ± SD of three mice per time point.