Caspase-1 is activated after HS/BFF. (A) Western blot images showing caspase-1 is activated (cleaved) in liver of WT (C57BL/6) mice at 6 h after HS/BFF. (B) Caspase-1−/− mice did not express IL18 at baseline or after HS/BFF, but NLRP3−/− mice expressed higher levels of IL18 than WT mice after HS/BFF measured by ELISA. (C) Western blot images with densitometry showing caspase-1 is activated (cleaved) in liver of NLRP3−/− mice at 6 h after HS/BFF. n = 3–6/group. Data show mean ± SEM. *P < 0.05, control versus HS/BFF; #P < 0.05 NLRP3−/− HS/BFF versus WT HS/BFF. C, control; ND, not detected; S, sham.