Increased remote organ damage after HS/BFF in caspase-1−/− mice. (A) ALT (left) and AST (right) in WT, caspase-1−/− and NLRP3−/− mice after HS/BFF. (B) H&E staining of liver from WT, caspase-1−/− and NLRP3−/− mice at 6 h after HS/BFF. (C) Western blot images and densitometry showing RIP-1 levels in whole liver cell lysates from WT and caspase-1−/− mice. n = 3–6/group. Data show mean ± SEM. *P < 0.05 WT versus caspase-1−/− HS/BFF.