Figure 6

Increased liver apoptosis of caspase-1^−/− mice compared with WT after HS/BFF. (A) Percentage of TUNEL-positive hepatocytes per high-power vision field in WT and caspase-1^−/− mice after HS/BFF. (B) Representative confocal microscopy images of liver sections showing TUNEL staining (green fluorescence, marked with white arrows) in WT and caspase-1^−/− mice after HS/BFF. Western blot images with densitometry analysis showing levels of caspase-9, caspase-12 and Bcl2 (C) and caspase-8 and cPARP (D) in whole-cell liver lysates of WT and caspase-1^−/− mice. C, control; S, sham. Images representative of three separate Western blots. n = 3–6/group. Data show mean ± SEM. *P < 0.05 WT versus caspase-1^{−/−; #}P < 0.05 versus control.