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Figure 2 | Molecular Medicine

Figure 2

From: Effect of Oxygen Levels on the Physiology of Dendritic Cells: Implications for Adoptive Cell Therapy

Figure 2

DCs differentiated and activated under atmosO2 and physO2 conditions show similar levels of surface molecule expression. Immature DCs were differentiated at atmosO2 and physO2 for 5 d. Subsequently, DCs were exposed to 10 ng/mL LPS (LPS-DC), a cytokine cocktail (CyC) containing IL-1β/TNF-α/PGE2 (CyC-DC) or media only (Im-DCs) for 48 h. DCs were collected, stained and analyzed by flow cytometry for the expression of the depicted surface molecules (A). Data shown are the MFI ± SD from three independent experiments using DCs from different donors. (B) The percentage of CD83-positive DCs. Data shown are mean ± SD from three independent experiments using DCs from different donors. (C) Data shown are the MFI ± SD from five independent experiments using DCs from different donors.

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