Figure 3
From: Effect of Oxygen Levels on the Physiology of Dendritic Cells: Implications for Adoptive Cell Therapy
Effect of oxygen levels on the cytokine profile of immature and mature DCs. (A) DCs were differentiated and activated as above with LPS or CyC. Cell culture supernatants were collected at 48 h, and the presence of the indicated cytokines and chemokines was measured by ELISA. Results shown are mean ± SD of six independent experiments using DCs from different donors. *Statistically significant difference; P < 0.05, paired Student t test. (B) Immature DCs differentiated at physO2 showed higher secretion of IL-12 when stimulated with CD40L. DCs were differentiated and LPS matured at both oxygen levels and then were cocultured with HeLa (data not shown) or HeLa CD154 cells for 12 h. Sups were collected and IL-12p(70) was measured by ELISA. Results are of six independent experiments using DCs generated from different donors. n.d., IL-12 was not detected in those samples.