Figure 1

(A) Gene expression analysis by microarray in human skeletal myoblasts (undifferentiated mononucleated cells, cultured in growth medium—15% FBS) and human skeletal myotubes (preformed myotubes, cultured in differentiation medium—2% horse serum) exposed to the HDACi TSA (50 nmol/L) for 24 h. contr.: control. (B) Representation of categories of genes induced by TSA in human skeletal myoblasts. (C) List of common genes found upregulated upon TSA treatment in both human skeletal myoblasts (exposed to TSA for 24 h in growth medium) and in mouse C2C12 myoblasts (exposed to TSA for 24 h in growth medium and then incubated in differentiation medium, without TSA). (D) Distribution of epigenetic marks (H3K4me3 and H3K27me3) in TSA-induced genes in human skeletal myoblasts.