Figure 3

Effect of pHBSP on lung injury and inflammation. Lung histological sections taken from a sham-operated rat with inflammatory cell infiltration grade 1 and no observable alveolar septa thickening (A); a rat subjected to HS with inflammatory cell infiltration grade 3 and widespread alveolar septa thickening (B); and a rat subjected to HS and administered 1 µg/kg pHBSP 60 min after the onset of resuscitation with inflammatory cell infiltration grade 2 and focal alveolar septa thickening (C). Hematoxylin and eosin, magnification × 100, Figures are representative of at least three experiments performed on different days. Effect of pHBSP on lung inflammatory cell infiltration (D), alveolar septal thickening (E) and ICAM-1 expression, corrected for the corresponding β-actin content and normalized using the related sham band (F), after sham operation (Sham, n = 3) or HS (HS + saline, n = 3; HS + pHBSP 60 min after resuscitation [1 µg/kg 60 min after the onset of resuscitation), n = 3). Data represent mean ± SEM for n observations, ⋆P < 0.05 HS + saline.