Effect of Deptor KD in C2C12 myoblasts. (A) Representative Western blot of Deptor protein in control (scramble) and Deptor KD myoblasts. (B) Quantification of Western blot data. Values are means ± SE; n = 12 per group. P< 0.05 compared with time-matched scramble control values. (C) Real-time PCR showing decreased Deptor mRNA in myoblasts transfected with lentiviruses targeting Deptor. rpL32 served as the internal reference control gene, and its expression did not differ between control and Deptor KD cell (Scramble control = 100 ± 5; Deptor KD = 107 ± 7; P= NS). Values are means ± SE; n = 12 per group. P< 0.05 compared with time-matched scramble control values. (D) Effect of Deptor KD on myocyte protein synthesis. Values are means ± SE for n = 12 for each condition. For bar graphs, *P< 0.05, compared with control values. (E) Effect of Deptor KD on mTORC1 and mTORC2 signaling in C2C12 myoblasts. Representative Western blots for protein substrates involved in the mTORC1 and mTORC2 complex mediated signal transduction. Where absent, standard error bars are too small to be visualized.