Effect of IGF-I and AICAR on scramble (control) and Deptor KD C2C12 myoblasts. Cells were transfected with scramble and Deptor KD-containing lentiviral particles and incubated with vehicle (control), IGF-I (100 ng/mL; 1 h) or AICAR (2 μmol/L; 8 h) and labeled with 35S-methionine. (A) Protein synthesis in myoblasts. Values are mean ± SE for n = 8–10 for each condition. Means not sharing the same superscript (a, b, c and d) are significantly different (P < 0.05). For quantification, data were normalized to scramble control values. (B) Representative Western blots for various total and phosphorylated proteins, where cells were treated as described above, except that the isotope was omitted. The blot is representative of at least three independent experiments with two to four replicates per experiment.